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文献和实验Procedures for In Vitro Production of Bovine Embryos
hood 100 ml pipet tips and pipettor OMM + supplements Mineral oil 60 x 15 petri dishes Plastic pasteur pipet 3-4 bottles of OCM + supplement (warmed @ 38.5°C) 1 L saline + Pen/Strep (warmed @ 38.5°C)Preparation for Oocyte Collection Scalpel Petri dishes
Double immunohistochemistry for BCL6 and PNA for Germinal Center detection.
chamber, at RT overnight . Rabbit anti BCL-6 (Santa Cruz Biotechnology, Santa Cruz, CA: Bcl-6 (N-3): sc-858, use at 0.5 µg/ml) 5- Wash twice in TBS-T. 6- add the biotin-conjugated secondary antibody (100 to 200 µl) and incubate for 45 min
" Solutions. 1. A molar solution is one in which 1 liter of solution contains the number of grams equal to its molecular weight. Ex. To make up 100 ml of a 5M NaCl solution = 58.456 (mw of NaCl) g x 5 moles x 0.1 liter = 29.29 g in 100 ml
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