赛默飞世尔Thermo Fisher PIPETTE TIP

Natural History Museum Protocol for EST sequencing from lamb

 (1) Core out a single, well separated plaque from the agar plate using a 200µl pipette tip and place it into 500µl of SM buffer in a 1.5ml microtube: SM buffer 5.8g of NaCl 2.0g of MgSO4.7H2O 50ml of 1M Tris HCl (pH 7.5) 5ml of 2% Gelatin

Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid

. If duplicates are used, transfer culture fluid from the duplicate block to the first block. Spin again, decant and drain. 3. Resuspend each pellet in 300ul of ice cold Qiagen buffer R1 with RNAse A (0.2 mg/ml) and RNAse T1 (100 units/ml). Let rest for 10

Kingfisher Flex 96 Plant DNA High Pure Protocol

for pipette 7. Ice Bucket 8. 1.5 or 2.0 mL microcentrifuge tubes, sealed deep-well plate or capped microtube rack for sample disruption 9. Kingfisher 96 or Kingfisher Flex 96 with Deep Well Magnet 10. 4 Deep Well Kingfisher 96 Plates