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大量
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两年
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科邦兴业(北京)科技有限公司
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盒
VS0231Vivaspin 2 50,000 MWCO PES 25/BOX
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文献和实验Studies of the Ubiquitin Proteasome System
( protocol 44 ) 50 mM MG132 (Calbiochem, Boston Biochem, or Biomol) in DMSO 10% (v/v) acetic acid/25% (v/v) methanol Basic Protocol 4: E3 Auto‐Ubiquitylation Materials Glutathione‐Sepharose (GS
Gel-purified, agarased YACs for yeast transformation
3TTK25, 30000 MWCO). Protocol : 1. CHEF gel (1% low-melt SeaPlaque agarose, 0.5X TBE, ca. 0.7cm thick). Load 4 X 2/3 of a plug per gel. Use same switching time as with regular agarose, but run 1.5X as long (e.g., for 600 kb
of linker histone required to saturate the nucleosome core array. The protocol given below describes the reconstitution using a DNA array containing 25 tandem repeats of 197bp 601 DNA (197bp x 25). The protocol is essentially split into two sections
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