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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 保修期:
两年
- 现货状态:
现货
- 供应商:
科邦兴业(北京)科技有限公司
- 规格:
盒
180F03---------ESartolab® RF 250;sterile;0.45μm;PES;12St
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文献和实验101 culture in 50 ml of 2xTY, as above. 2. Using sterile toothpicks, transfer individual M13 plaques into 12 X 75 mm Falcon tubes containing 1 ml early log phase cell cultures, and incubate for 4-6 hours at 37degC with shaking at 250 rpm. (Growth
Methods for DNA isolation(DNA分离方法)
of bacteria harboring the plasmid or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100μg/ml)and incubate at 37deg C 8-10 hours with shaking at 250 rpm
5.0 65 260 8.0 45 160 12.0 20 70 20.0 12 45 2. Migration of marker dyes in polyacrylamide denaturing gels Gel % Bromophenol blue (BP) Xylene cyanole (XC) 5.0 35 130 6.0 26 106
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