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大量
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年
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科邦兴业(北京)科技有限公司
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VS0201Vivaspin 2 10,000 MWCO PES 25/BOX
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文献和实验Studies of the Ubiquitin Proteasome System
1 (see recipe ) 10 mg/ml ubiquitin Substrate, expressed with 35 S‐label in reticulocyte or wheat germ lysates (25,000 to 100,000 cpm; see protocol 40 ) E3, expressed as a GST fusion
), which are sealed at one end with a silicone bung, and at the other with a section of dialysis membrane (7’000 MWCO). All the dialysis vessels are then placed inside another dialysis bag, filled with ~20ml reaction buffer (2M NaCl, 10mM TEA-HCl pH7.4, 1mM
Gel-purified, agarased YACs for yeast transformation
3TTK25, 30000 MWCO). Protocol : 1. CHEF gel (1% low-melt SeaPlaque agarose, 0.5X TBE, ca. 0.7cm thick). Load 4 X 2/3 of a plug per gel. Use same switching time as with regular agarose, but run 1.5X as long (e.g., for 600 kb
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