Sartorius赛多利斯VS0151Vivaspin 50

【资源】蛋白质浓缩，两个小tips

, a MWCO of 12,000 to 14,000 is appropriate, whereas a MWCO of 3500, 2000, or even 1000 is appropriate for peptides. Most dialysis membranes are made of derivatives of cellulose. They come in a wide variety of MWCO values, ranging from 500 to 500,000

Chromatin IP (CHIP assay)

PCR purification kit) purify DNA through Qiaquick column elute DNA into 50 µl H2 O use 0.5 - 1 µl per 25 µl PCR reaction:      1 x 95 °C, 2 min      21 x 95 °C, 30 sec; 60 °C, 30 sec; 72 °C, 1 min      1 x 72

RNA实验方法

) but this material is no longer available. RNA that is 50 bp DNA fragments, require alkaline transfer conditions and may have higher backgrounds.Hoeffer sponges are 6?x 9?and the gel must be cut to fit these dimensions.The Hoeffer gel box can blot 4 gels