sartorius赛多利斯14053--47----RDNE

Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period

100. Add 10 ml of 1 M Tris, 109.54 gr of sucrose, 1.01 gr MgC12, adjust pH to 8.0 and finally add 10 ml of Triton X-100 to 800 ml of distilled water, and make up to 1 liter with distilled water. Autoclave at 15 p.s.i. for 10 min. Sugars at high

ChIP-Chip E. coli

Bacillus subtilis , Caulobacter crescentus and Mycobacterium tuberculosis . Because E. coli can be grown to high cell densities relative to eukaryotes, it is possible to generate sufficient DNA to label without using a PCR-based method. This method uses

High Efficency Yeast Plasmid Rescue

45 seconds. Place column in a clean tube and add 50 µl of buffer EB. Let sit for 1 min. then spin for 1 min. For efficent transformation into coli use 1-2 µl of this prep per aliquot of E. coli using the 90sec heat shock