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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70312318199
中文名称:
英文名称:X50 AUTOCLAVE INDICATOR 3M ATTEST BIOLOGICAL, steam, rapid cap colour brown, EN866, ISO 11138,
货期:热销品现货,其他请咨询




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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
temperature. [E buffer: 50 mM Tris-Cl pH 8.0, 300 mM NaCl, 5 mM EDTA, pH 8.0, 2 SDS; autoclave and add 1 mM ATA and 14 mM ß-mercaptoethanol. ATA=aurintricarboxylic acid, ammonium salt (Sigma #A0885, St. Louis, MO)] 5. Thaw the powder in E buffer in 42°C
. All items should have indicator tape. Separate liquids from solids and autoclave separately. Make sure lids on all bottle are loose. Do not crowd large number of items in tray- in order for all items to reach the appropriate temperature, one must allow
A quick RNA mini-prep for Neurospora mycelial cultures
tubes from collapsing. 8. Pass supernatant through 50 micron Miracloth (Calbiochem #475855, La Jolla, CA) in a funnel into fresh Sarstedt tube. 9. Measure volume of average-sized sample. Add 0.5 vol. 8 M LiCl, mix and stand at 4°C overnight.
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