赛默飞世尔Thermo Fisher 750 N 500µL

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

% SDS lysis buffer into 500 µl of supernatant (final 1% SDS). Mix gently. This step increases the SDS conc. in the sample to 1%, which is necessary to make the length of chromatin DNA short enough for ChIP (200 � 1000 bp) by sonication

RNA In Situ Hybridization of Dissected Gonads with Digoxygenin-Labeled Probes

tablet (#B5655) ; Dissolve one tablet in 10 ml staining solution. If there is a non-specific background signal, you can dilute more (up to 20 ml).       Culture tubes : Available from Fisher as 6 mm (ID) x 50 mm (length

单细胞基因表达分析

triethylamine acetate (TEAA, Cat. No. 400613) were from Applied Biosystems and the anhydrous acetonitrile (Cat. No. 40-4050-50) was from Glen Research. Trifluoroacetic acid (TFA, Cat. No. BP618-500) was from Fisher and triethylamine (TEA, Cat. No. T-0886