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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310136412
中文名称:
英文名称:X960 Cryovial Cryobank 96 well format for protein/ cell storage coded 1mL sterile (supplied as 10
货期:热销品现货,其他请咨询




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文献和实验overnight (12-16 hours) at 37degC with shaking at 250 rpm. 4. Add 12.5 ml of sterile glycerol for a final concentration of 20%, and distribute the culture in 1.3 ml aliquots into 12 X 75 mm Falcon tubes. 5. Store glycerol cell stocks frozen
and further incubate overnight (12-16 hours) at 37℃ with shaking at 250 rpm. 4. Add 12.5 ml of sterile glycerol for a final concentration of 20%, and distribute the culture in 1.3 ml aliquots into 12 X 75 mm Falcon tubes. 5. Store glycerol cell stocks frozen at -70℃
lysate to a fresh tube. Depending on the cell type you are using, this may or may not be necessary - however, it never hurts to do it. 7.) Determine protein concentration by whatever method you’re used to. 8.) Typically, I use 150-200μg
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