赛默飞世尔Thermo Fisher X4000 Cap A

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

  (1)  Remove vitelline membrane from stage 21-23 embryos (n = 300). Collect head tissues (about anterior 1/4 or 1/3 of the embryo) in 1x MBS. (2)  Fix the tissues in 0.5x MBS/1% formaldehyde (4-5 ml in a screw cap glass vial) for 15 min

Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shea

so that a 75 x 25 x 1 mm microscope glass slide will sit flush in the chamber.     7)      Create a recess for an O-ring around the underside of the top and bottom plates such that they are spaced apart by 0.02". Insert the rubber O-rings.     8)   

常用缓冲液配制－2

into several small screw-cap jars (~50 ml capacity) Store at room temperature  20 x Energy Regeneration System* 150 mM creatine phosphate (Boehringer #127574) 2 ml of 100 mM MgATP Stock (20 mM ATP, 20 mM MgSO4 ) 40ml 0.5 M EGTA (2 mM EGTA)