赛默飞世尔Thermo Fisher FILTERS IMM
OBILON-NY+ TRANSFER MEMBRANES ROLL 30cm x 3m 0.45µm pore size 货号：T_70310439820- ￥1200 - 24000
- Thermo Fisher
- 美国/芬兰/中国/韩国/墨西哥
- T_70310439820
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310439820
中文名称：
英文名称：FILTERS IMMOBILON-NY+ TRANSFER MEMBRANES ROLL 30cm x 3m 0.45µm pore size
货期：热销品现货，其他请咨询
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文献和实验Screening Colonies by Hybridization with Radiolabeled Probe
onto neutralizing solution. Transfer for 5 minutes onto 2 x SSC. Transfer onto a dry sheet of 3MM paper, let air dry for at least 30 minutes at room temperature. Sandwich the filters between sheets of 3MM paper. Fix the DNA to the filters
filters to tray and cover with typically a few hundred ml of 5x SSPE. Incubate in 65℃ oven 30-45 min, gently shaking every 10 min. Discard this 5x SSPE in 32 P liquid waste container. Transfer filters to 2x SSPE for 20 min at room temperature, shaking
Screening of cDNA and genomic libraries
to 15 ml tube. Add 300,000 pfu from the library, x ul. Incubate 30 min. at 37 C. Transfer 300 ul of phages / cells to 7 ml topagarose (tubes with topagarose is kept at 50 C). Invert the tube a couple of times and pour
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