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999
- 供应商:
北京泽平
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- 保修期:
1年
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EA
货号:T_70311525664
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英文名称:X2 Protein A Mag Sepharose Xtra, particle size 37µm to 100µm 1mL Store in fridge at 4°C
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文献和实验Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
in 37°C shaker incubator (usually 6-8h) Add 4.5 ml of a sterile glycerol-media solution (5ml of autoclaved 80% glycerol solution + 4ml LB + 9µl 1000x tetracyclin stock). Mix well. Aliquot into sterile centrifuge tubes (1ml/tube
Studies of the Ubiquitin Proteasome System
phosphatase inhibitors and 50 µM MG132 (or lactacystin) Anti‐E3 or anti‐substrate antibody Nondenaturing Triton X‐100 wash buffer (see recipe ) Tris‐buffered saline (TBS
solution, e.g. 0.1% Triton X-100 at 37°C for one minute. Re-equilibrate immediately with at least 5 bed volumes of binding buffer. 8. Storage Benzamidine Sepharose 6B should be stored at 4�8°C in the presence of a suitable bacteriostatic agent, e
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