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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310703141
中文名称:
英文名称:X100 Filter disc Millipore nylon 25mm diameter 20µm mesh opening (pack of 100)
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文献和实验Analys of Genomic DNA by Southern Hybridization
above) 3.Add 0.1 volume (10µl) 3 M sodium acetate, mix well. Add 2.5 volumes ice-cold 100% ethanol. Precipitate at -80℃ for 1 hour . Spin in microfuge at high speed for 20 minutes. Discard supernatant, air-dry for 5 minutes. Resuspend in 15 µl 10mM Tris pH
Screening Colonies by Hybridization with Radiolabeled Probe
Hybridization oven Hybridization bottles Nylon mesh pieces Waterbath at 100°C Kimwipes Reagents: Prewashing solution , 50 ml per bottle, (5 x SSC, 0.5% SDS, 1 mM EDTA pH 8.0) for 50 ml: 12.5 ml 20 x
Analys of Genomic DNA by Southe
as described above) 3.Add 0.1 volume (10µl) 3 M sodium acetate, mix well. Add 2.5 volumes ice-cold 100% ethanol. Precipitate at -80℃ for 1 hour . Spin in microfuge at high speed for 20 minutes. Discard supernatant, air-dry for 5 minutes. Resuspend in 15 µl 10mM
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