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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:3466AS
中文名称:
英文名称:1.5ml Screw Cap Tube,Knurl, NonSkirted,Amber, E-Beam Sterile, tube only no cap
货期:热销品现货,其他请咨询




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文献和实验Materials2 ml size Sarstedt screw cap tubesBIO-RAD Disposable Chromatography Column (BIO-RAD cat. #732-6008)Oligo dT celluloseRNase-free water5.0 M NaCl0.5 M EDTA1.0 M Tris, pH 7.410% SDS70% EtOHBefore starting protocol1. Measure out 75 mg of oligo
Competent cells, calcium chloride method, E. coli, description
. Grow 5 ml overnight of the strain in 2 X YT in a standard container. Screw cap tight and lie flat on the shaker for good aeration. Dilute overnight culture 1/100 - 1/200 to 25, 50 or 100 ml 2 X YT broth in flasks 5 - 10 X culture volume (i.e
Broth culture of E. coli Tubes of nutrient broth (9.9 ml each) Nutrient agar plates Sterile transfer pipettes (1.0 ml) Quebec colony counter (Optional) Procedure 1. Obtain a broth culture of E. coli and carefully mix
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