赛默飞世尔Thermo Fisher BEVELED ORI
FICE PIPETTE TIP 1-300UL NATURAL STERILE 96 TIP RACK HINGED 5M/CS 货号：T108RS-Q- ￥1200 - 24000
- Thermo Fisher
- 美国/芬兰/中国/韩国/墨西哥
- T108RS-Q
- 2025年12月24日
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货号：T108RS-Q
中文名称：
英文名称：BEVELED ORIFICE PIPETTE TIP 1-300UL NATURAL STERILE 96 TIP RACK HINGED 5M/CS
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文献和实验Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
reaction will require 160-300ng of template. 1. Streak clone stock to single colony on LB (+antibiotic) plate. Inoculate 1ml LB (+antibiotic) culture with single colony, grow overnight at 37?C. Using 3 ul of (overnight growth) single colony
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
a multichannel pipette disperse cells as before, transfer 25 ul into 96 well plate containing 10% DMSO in medium. Then using a Gilson and a sterile tip transfer remaining cells to a 24 well plate with 1 ml medium with LIF (no feeders) in each well, 24 wells
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
), remove medium using aspirator and sterile pasteur pipette, wash each well with 0.1 ml PBS, aspirate, wash with 0.1 ml PBS/EGTA, aspirate, add 40 ul TRYPSIN/EDTA, using a multichannel pipette disperse cells as before, transfer 25 ul into 96 well plate
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