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文献和实验96 Well Mating Protocol platewise
on the XGAL plate 2. Streak recipient plasmid (pMSCV) on LB Spec50 CB100 0.2% glucose OR YEGlu spec50 cb100) Grow at 30oC overnight Protocol 1. Inoculate 5 ml single colony culture of recipient, and a 1:100 donor culture in 500 ul/well 96 well culture box
96 Well Mating Protocol platewise
, 100ul recipient pool is aliquotted to is added to each well of Costar U-bottom 96 well plate. Then each well gets either 1-2ul of donor overnight culture. Donor dilution: Acceptable donor OD600 range is 0.060 to 0.300 . Measure the cultures straight
E-Z 96 Plant DNA Protocol-Vacuum Manifold Processing
the vacuum to elute the DNA into collection plate. TIP: 100 ul water or TE buffer is sufficient to elute up to 85% of the DNA from each well of the E-Z 96 DNA plate. A second elution step with same 100 ul elutate containing DNA, reheated to 65°C
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