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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:584875
中文名称:
英文名称:10ul Micro Pipet Tip non-sterile 10 trays/pk
货期:热销品现货,其他请咨询




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文献和实验ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
5 Labeling of RNA for Microarrays #2 Modified to use Genisphere Sub-micro kit **** CAUTION *** Protocol under construction! In microfuge tube combine: ("Primer Mix", 10ul) 2ul total RNA (~.5 ug) 3ul RT Primer (0.2 pmole… "Vial 2") 5ul RF-H2
RNA purification using TRizol--TRIZOL法提取RNA
microcentrifuge tubes.b.Centrifuge for 1 minute to pellet the cells.c.Pour off the supernatant.d.Add 1 ml of TRIzol or TRI reagent to the tubes.e.Lyse cells by repetitive pipetting.f.Centrifuge homogenate at 12000 x g for 10 minutes at 4℃.g.Transfer the homogenate
:1) and gently shake for 2 hrs. Centrifuge at 2,800 rpm for 30 min. Collect supernatant using a sterile transfer pipet (IWAKI 7801-002, cut off 3 cm from the tip). Add 2 ml 10% CTAB solution and mix gently. Add 20 ml 24:1 chloroform : isoamylalcohol and gently
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