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文献和实验Production of L. pneumophila Monospecies Biofilms in a Low-Nutrient-Concentration Medium
In aquatic environments such as water distribution systems, Legionella pneumophila persistence is �correlated to the presence of a biofilm. The method described here permits the formation of a monospecies L. pneumophila biofilm in microplates
Predicting Peptide Retention Times for Proteomics
. Krokhin, O.V. 2006. Sequence‐specific retention calculator. Algorithm for peptide retention prediction in ion‐pair RP‐HPLC: Application to 300‐ and 100‐A pore size C18 sorbents. Anal Chem. 78:7785‐7795
in a low speed centrifuge at 2000 rpm for 5 minutes. If I ligate 100 ng of blunted dephosphorylated vector to 50 ng of a blunt 500 bp PCR in a 30 µl reaction, I'll usually transform 5 ul into cells and plate out the whole tranformation, I normally
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