Sartorius赛多利斯16553----------KM

【交流】关于NEB的T4 DNA连接酶和快速连接试剂盒的常见问题及解答

液： 1X T4 DNA Ligase Buffer: [50 mM Tris-HCl, 10 mM MgCl2, 10 mM DTT, 1 mM ATP, 25 µg/ml BSA, (pH 7.5 @ 25°C)]。推荐DNA浓度(0.1 - 1 µM 的5′末端)。 使用注意：ATP是反应必须的辅因子。这一点与要求NAD作辅因子的大肠杆菌DNA连接酶不同。 如在反应体系中补加1 mM的ATP，连接反应还可以在NEB提供的四种限制性内切酶缓冲液(NEBuffers)或在T4 DNA多聚

Screening lambda cDNA or genomic libraries

need 6.6 ml top agarose (use a bigger tube and a 50-55° water bath).to titer (on 100 mm plates):Put various dilutions of phage in 100 µl 1X phage buffer.Always do a no phage control! This will help you distinguish between dilutions that give you no phage (look

DNA疫苗实验方法

you want). See "Plasmid DNA Preparation" protocols for detailed information. Each TA muscle is injected with 50 µl of DNA solution. To inject plasmid DNA use a 27GX3/4" (0.4x20mm) needle attached to a 1 ml tuberculin syringe. A piece of polyethylene