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- 2025年07月15日
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HTTP04700
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HTTP04700 MilliporeIsopore 表面滤膜，聚碳酸酯，亲水，0.4 µm，47 mm，白色，光面
| 描述 | |
|---|---|
| 产品目录编号 | HTTP04700 |
| 商名 |
|
| 描述 | Isopore 表面滤膜，聚碳酸酯，亲水，0.4 µm，47 mm，白色，光面 |
| 背景信息 | The Isopore™ membrane is a polycarbonate, track-etched screen filter recommended for all analyses in which the sample is viewed on the surface of the membrane. Isopore™ membrane offers distinct advantages for the analysis of airborne contaminants and other particles using optical or electron microscopy. The Isopore™ membrane is composed of polycarbonate film, which has a smooth, glass-like surface for clearer sample observation. The unique manufacturing process of the membrane ensures a precise pore diameter and a consistent pore size for accurate separation of samples by size. Matched-weight filters are not usually required because of low, constant tar and ash weights. Features & Benefits: •Membrane structure retains particles on the surface, simplifying counting and analysis •Isopore™ membranes do not stain, resulting in low background interference •Non-hygroscopic, allowing for rapid drying and reduced sample analysis time •Translucent material does not require clearing for transmitted light microscopy; also available in brown variety Applications: Air Monitoring, Epifluorescent Microscopy, Chemotaxis Assays |
| 产品信息 | |
|---|---|
| 过滤器代码 | HTTP |
| 过滤器颜色 | 白色 |
| 最高工作温度 | 140 °C (possible reduction in flow rates may occur after autoclaving) |
| 过滤器类型 | 表面滤膜 |
| Quality Level | MQ400 |
| 应用 | |
|---|---|
| 应用 | 0.4 µm pore size, hydrophilic polycarbonate membrane, 47 mm diameter |
| 生物信息 | |
|---|---|
| 媒质 | Isopore® |
| 可湿润性 | 亲水性 |
| 理化信息 | |
|---|---|
| 折射系数 | 1.6 |
| 孔径 | 0.4 µm |
| 空气流量 | ≥7 L/min @ 10 psi |
| 23°C时的泡点 | ≥2 bar |
| 重量分析的提取物（%） | < 1% |
| 孔隙率% | 10%-20% |
| 水流量 |
|
| 尺寸 | |
|---|---|
| 过滤器表面 | 平纹 |
| 厚度 | 10 µm |
| 过滤器直径 (⌀) | 47 mm |
| 材料信息 | |
|---|---|
| 化学 |
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| 包装信息 | |
|---|---|
| 数量 | 100 |
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文献和实验Site-directed hydroxy radical mapping of nucleosome positions in vitro
12). Mapping Reaction A typical sample for mapping has a total volume of 10-20µl and contains 0.2-0.5µM reconstituted nucleosome, 50mM Tris-Cl pH 7.5 and appropriate monovalent salt. After desired experimental manipulation, place sample
后，用玻棒赶出所有的气泡。 3）用一把新的解剖刀或切纸刀裁一张硝酸纤维素滤膜（Schleicher ＆Schuell BA85或与之相当的产品）。滤膜的长度和宽度应分别比凝胶大1mm， 接触滤膜时须戴手套或用平头镊子（例如Millipore镊子），用有油腻的手接触过的硝酸纤维素滤膜不易浸温。 4）将硝酸纤维素滤膜浮在去离子水表面，直至滤膜从下向上湿透为止，随后用20xSSC浸泡滤膜至少5分钟，用干净的解剖刀片切去滤膜一角， 使其与凝胶的切角相对应。不同批号的硝酸纤维膜，其浸湿
Anti-GFP/BrdU In Situ Immunostaining
Materials 24 well dishes Millicell 0.4 µm inserts (Millipore PICM01250) - coated with fibronectin Culture medium PBS Fixative: 4% paraformaldehyde in PBS (pH 7.6) Rabbit anti-GFP (Molecular Probes, A-11122) FITC-goat anti-rabbit
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