Polyclonal Antibody to X-linke

Flow Cytometric Measurement of SLAM-Associated Protein and X-Linked Inhibitor of Apoptosis

of immune homeostasis, SLAM-associated protein (SAP) and X-linked inhibitor of apoptosis (XIAP). These proteins are defective in patients with the immune deficiency X-linked lymphoproliferative disease (XLP), due to mutations in the SH2D1A and XIAP/BIRC

Introduction to Antibodies - Enzyme-Linked Immunosorbent Assay (ELISA)

for 2 hrs. to overnight in a humid atmosphere at room temperature. Note: Sodium azide is an inhibitor or horseradish peroxidase. Do not include sodium azide in buffers or wash solutions if an HRP-labeled antibody will be used

CO-IMMUNOPRECIPITATION ANALYSIS OF PROTEIN-PROTEIN INTERACTIONS

into a 15 ml tube. For immunoprecipitation, add 10 ml (polyclonal serum) or 10 mgs (monoclonal antibody) to 1 ml of extract. Incubate with end-over-end mixing for 2 hours at 40 C. Save 50 ml of each cell extract and add 50 ml of 2X SDS-PAGE sample buffer