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- 详细信息
- 文献和实验
- 技术资料
- 库存:
1000
- 英文名:
Stampwell-V-500
- 保质期:
长期
- 供应商:
北京博蕾德生物科技有限公司
- 保存条件:
常温
- 规格:
1 Unit Pack
凝胶V型孔制备(3D细胞成像),Stampwell只需一次即可在水凝胶中压印V形孔。没有什么比把你的样品加载到井里和图像更重要的了。Stampwell形状已针对球体状细胞和类器官的成像进行了优化。
Stampwell stamps imprint arrays of wells in hydrogels in just one time. Nothing more to do than load your samples into the wells and image. The Stampwell shapes have been optimized for the imaging of spheroids and organoids.
V Shape 300µ:
. Number of pins: 42
. Depth of the well: 1 mm
. Well bottom diameter: 300µm - Shape: circular
. Well upper side: 1mm*0.5mm - Shape: rectangular
V Shape 500µ:
. Number of pins: 42
. Shape of the pins: V
. Depth of the well: 1 mm
. Well bottom diameter: 500µm - Shape: circular
. Well upper side: 1mm*0.5mm - Shape: rectangular
Protocol overview
1. Pour liquid agarose (or other hydrogel)
2. Place the stamp
3. Reticulate the hydrogel
4. Remove the stamp

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文献和实验Original publication:
Stampwell has been originally developed by Gaelle Recher and published in Scientific Reports:
Alessandri K, Andrique L, Feyeux M, Bikfalvi A, Nassoy P, Recher G. All-in-one 3D printed microscopy chamber for multidimensional imaging, the UniverSlide. Sci Rep. 2017 Feb 10;7:42378. PMID: 28186188; PMCID: PMC5301227.
Citations:
Merdrignac C, Clément AE, Montfort J, Murat F, Bobe J. auts2 Features and Expression Are Highly Conserved during Evolution Despite Different Evolutionary Fates Following Whole Genome Duplication. Cells. 2022 Aug 30;11(17):2694. PMID: 36078102; PMCID: PMC9454499.
Sahai-Hernandez, P., Pouget, C., Eyal, S., Svoboda, O., Chacon, J., Grimm, L., Gjøen, T., & Traver, D. (2023). Dermomyotome-derived endothelial cells migrate to the dorsal aorta to support hematopoietic stem cell emergence. eLife, 12, e58300.
105重悬的细胞,300 g离心5 min,弃上清。加入500 μL稀释成1 ×的Annexin V Binding Buffer工作液重悬细胞。 细胞悬液中加入5 μL的荧光素标记-Annexin V和5 μL的细胞核染色液。 轻柔涡旋混匀后,室温避光孵育15~20 min。 反应完成后立即上机检测。如不能及时检测,请于冰上避光静置并于1 h内完成检测。 检测 1)流式细胞仪检测 处理好的样本在流式细胞仪上检测。 2)荧光显微镜分析 取一滴用荧光素-Annexin V/细胞核染色液双染的细胞
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