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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
- 库存:
货期:1-2天
- 供应商:
MedChemExpress LLC
- CAS号:
67-45-8
- 规格:
25 mg/50 mg/100 mg
| 规格: | 25 mg | 产品价格: | ¥350.0 |
|---|---|---|---|
| 规格: | 50 mg | 产品价格: | ¥530.0 |
| 规格: | 100 mg | 产品价格: | ¥800.0 |
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文献和实验: "Quickie" PCR is quite feasible: eg, [94oC 30 sec / 45oC 30 sec / 72oC 30 sec] x 30, for short products (200 - 500 bp). YOU CAN USE GLYCEROL IN THERMAL CYCLER REACTION TUBE HOLES TO ENSURE GOOD THERMAL CONTACTS DON'T RUN TOO MANY CYCLES: if you don't
Thermal Cycling Profile for Standard PCR
is normally carried out at 72°C. Taq DNA Polymerase can add approximately 60 bases per second at 72°C. A 45-second extension is sufficient for fragments up to 1 kb. For extension of fragments up to 3 kb, allow about 45 seconds per kb. However, these times
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
ug/ml KAN, 50ug/ml Spec, 25ug/ml Chlor For pBI types in gv3101 - 50ug/ml KAN, 25ug/ml GEN 2. Remove 1 ml cells to two microfuge tube 3. Centrifuge 45 sec and remove the supernatant with aspiration 4. Add 1 ml cells more to both tubes
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