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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
- 英文名:
HOE-498 (Standard)
- 库存:
货期:询盘
- 供应商:
MedChemExpress LLC
- CAS号:
87333-19-5
- 规格:
5 mg/10 mg/25 mg/50 mg/100 mg
| 规格: | 5 mg | 产品价格: | ¥320.0 |
|---|---|---|---|
| 规格: | 10 mg | 产品价格: | ¥480.0 |
| 规格: | 25 mg | 产品价格: | ¥860.0 |
| 规格: | 50 mg | 产品价格: | ¥1300.0 |
| 规格: | 100 mg | 产品价格: | ¥2000.0 |
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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
, gv3101 - 25GEN 2. Transfer O/N culture to 200ml YEP in a sterile 500ml flask and shake at 250rpm until the OD is 0.3 (4-5hrs) 3. Spin in sterile 50ml screw cap tubes 4C 5krpm 10‚. Check to make sure cells are pelleted, if not repeat at higher
real time sybergreen concentration-Real-Time PCR
you (I found them recently): - "A quantitative fluorescence-based microplate assay for the determination of double-stranded DNA using SYBR Green I and a standard ultraviolet transilluminator gel imaging system.", Vitzthum F, Geiger G, Bisswanger H, Brunner H, Bernhagen
AFLP: not only for fingerprinting, but for positional cloning
Preparation EcoRI-adapter = 5'-CTCGTAGACTGCGTACC (EcoRI-oligo.1) CTGACGCATGGTTAA-5' (EcoRI-oligo.2) - mix 1.7 µg EcoRI-oligo.1 and 1.5 µg EcoRI-oligo.2, 3 µl 10X One-Phor-All+,Q.S. to 60 µL w/ dH2O, heat to 95° C, and allow to cool to RT slowly
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