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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
- 英文名:
PFTrDA
- 库存:
货期:询盘
- 供应商:
MedChemExpress LLC
- CAS号:
72629-94-8
- 规格:
10 mg/25 mg/50 mg/100 mg
| 规格: | 10 mg | 产品价格: | ¥300.0 |
|---|---|---|---|
| 规格: | 25 mg | 产品价格: | ¥600.0 |
| 规格: | 50 mg | 产品价格: | ¥950.0 |
| 规格: | 100 mg | 产品价格: | ¥1050.0 |
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文献和实验More than 15 years after its initial description, the polymerase chain reaction (PCR) has become a standard molecular biology tool and is the most widely used method of nucleic acid amplification. The procedure is based
DNA Amplification Fingerprinting Protocol
. Amplifications are performed using the 96-well plates in a MJ Research thermal cycler for 35 cycles after an initial denaturation at 94C for 5 min and a final extension at 72C5 min. For arbitrary and mini-hairpin primers, each cycle consists of 5 sec at 94C, 20
【精华】vol 687 Chapter 4 巢式PCR侧翼序列测定实验
to template a 50 mlPfuTurbo?hot start PCR in (see Note 13) 1× reaction bufferincluding 300 mM dNTPs, 3.75 U PfuTurbo?DNA Polymerase, and 250 nM GSP1 and GSP4. Thermocyclingparameters used are: 40 cycles of 94°C for 1 min, 50°C for1 min, 72°C for 5 min
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