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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
BSA PDLA Particles
- 库存:
100
- 供应商:
百欧泰(biotyscience)
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文献和实验CELL ADHESION Procedure: 1) Dissolve/dilute coating substrate in ddH2O at 4 C. A common working dilution for the laminin-1 positive control and BSA (Sigma A8412) negative control is 40 ug/ml. For SN-peptide, use 20 uM (MW of SN
PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
, gelatin or BSA to 100ug/ml, and/or non-ionic detergents such as Tween-20 or Nonidet P-40 or Triton X-100 (0.05 - 0.10% v/v) (Innis and Gelfand, 1990)。 Modern formulations may differ considerably, however - they are also generally proprietary. PCR
Immunofluorescence / Confocal Microscopy Protocol
5. Wash 1x PBS/BSA. 6. Block 15 min 5% normal goat serum (NGS) in PBS/BSA. 7. Wash 1x PBS/BSA. 8. Diluted in PBS/BSA 60 min RT; 100 l per tube or section. 9. Wash 3x PBS/BSA. 10. Block 15 min 5% NGS in PBS/BSA. 11. Wash
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