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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Protein A PE Polypropylene Particles, 50 um
- 库存:
100
- 供应商:
百欧泰(biotyscience)
- 规格:
10 mg/ml10ml
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文献和实验Use of Flow Cytometric Methods to Quantify Protein‐Protein Interactions
diameter (Spherotech, SVP‐50‐5) Bead coupling buffer (BCB; see recipe ) Biotinylated RGS protein (see protocol
comb) Constant current power supply (500 V and higher) Clear, robust plastic foil (e.g., pieces of freezer bags or clear disposal bags—polyethylene or polypropylene, 40‐ to 50‐µm thick) Table
4 µg of antibody,400 µl of H2O,400 µg total protein to microcentrifuge tube. 2.Vortex and incubate at 4celsius degree for 1 Hr. 3.Add 10 µl 50% protein A : Agrose,vortex and incubate for 30 minutes at 4 ℃. 4.Centrifuge the agarose solution
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