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- 文献和实验
- 技术资料
- 英文名:
Protein G PE Polypropylene Particles, 15 um
- 库存:
100
- 供应商:
百欧泰(biotyscience)
- 规格:
10 mg/ml10ml
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文献和实验Western Blotting with Horseradish Pe
. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. POLYACRYLAMIDE GEL
Evaluating Modulators of “Regulator of G‐protein Signaling” (RGS) Proteins
), and for validation of candidate GAP?modulatory molecules with the single?turnover GTP hydrolysis assay. Curr. Protoc. Pharmacol. 56:2.8.1?2.8.15. © 2012 by John Wiley & Sons, Inc. Keywords: regulator of G?protein signaling (RGS) proteins
?equilibrium assays to ensure proper interpretation of the findings. Outlined in this unit are the most common experimental approaches for measuring allosteric effects on orthosteric ligand affinity at G protein?coupled receptors. There is also a discussion
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