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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
BSA PE Polypropylene Particles, 24 um
- 库存:
100
- 供应商:
百欧泰(biotyscience)
- 规格:
10 mg/ml10ml
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文献和实验1-2 μl (20-40 U is sufficient) Enzyme 2 1-2 μl (same as above) 100X BSA 1 μl Water to 50 μl total volume Note: Add enzymes LAST. Digest at 37 ℃ for 2-3 h (no longer!). 1.
T-cell dependent antigen specific in situ staining (Pe)
if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe
10X Buffer 5 ul (determine appropriate buffer from NEB catalog) Enzyme 1 1-2 ul (20-40 U is sufficient) Enzyme 2 1-2 ul (same as above) 100X BSA
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