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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 保质期:
6个月
- 库存:
100
- 供应商:
百欧泰
- 规格:
10 ml/20 ml
| 规格: | 10 ml | 产品价格: | ¥950.0 |
|---|---|---|---|
| 规格: | 20 ml | 产品价格: | ¥1500.0 |
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
reaction buffer can be stored for at least one year at room temperature in foil-covered container. X-gal stock(40X) 40 mg/ml X-gal in dimethylformamide Store at -200 C in foil covered glass container. Rinse many times in PBS
solution)to microcentrifuge tube. 2.Add 2.5x 10,000 particles of 1 uM Fluosphere beads. 3.Mix overnight at 4 ℃ with 200 µl 5mg/ml 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide. 4.Wash with PBS,by centrifuging,repeat wash 3 times. 5.Resuspend the bead
WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos
% ethanol. Rinse with 125µl 75% ethanol. Let dry with caps open for 10 minutes. ) Resuspend in suitable volume of nuclease free water. Measure concentration. Set up transcription reaction 200ng DNA 2 µl 10
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