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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Protein A PET Particles
- 库存:
100
- 供应商:
百欧泰(biotyscience)
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文献和实验Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
test protein's open reading frame Modified vectors (available from Nick Loizos on request) of the pET expression system:
Experimental Applications: Human Acetylcholinesterase as a Model Nervous System Protein
AChE. At 10−4 M BW, endogenous oocyte AChE activity (Gundersen and Miledi, 1983) was only 50% inhibited, whereas rHAChE was essentially 100% inhibited at this concentration. A similar differential sensitivity of the amphibian and mammalian enzymes
Purification of KHC Motor Domain Protein
mM NaCl (5 x 1 mL fractions), then with HEM + 200 mM NaCl (8 x 1 mL fractions). The bulk of the protein starts to elute with 100 mM NaCl in the 2nd fraction. Add 5 microliters of 200 mM PMSF to each peak fraction. 9. Pool
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