PLLA Particles/PLLA微球, 12 um-B

pc12培养

processes可以长达500~1000um。若去除NGF的作用，24h内processes degenerate，72h内resume multiply。passage 达70代其生物学性质也无明显改变！（来自Greene LA 1976,73:2424-2428) 2.不知flask用生长基质包被了没有，若无，PC12细胞既可呈悬浮生长又可呈贴壁生长！ 3.国产的胎牛血清从质量上至多达到国外小牛血清的水平，本人建议楼主换用进口的FBS。本人应用PC12作转染试验，培养液为DMEM（high glucose

Coupling of Monoclonal Antibodies with Biotin

of the extracellular matrix that specifically promotes cell adhesion. Consequently, the streptavidin-cell-surface interaction causes high background in certain applications ( 12 ). Fig. 1.  Structure of biotin.

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

in the nucleus in human cell cultures we designed several RNA-biotin based pulldown assays which can be used alone or in combination with other known assays such as ChIP and Flag-tagged pulldown assays. Three protocols are explained