Phospho-γ-H2AX Antibody-Phosp

利用共聚焦显微镜实现 DNA 修复蛋白可视化

上聚集   利用 405 nm 激光对表达 GFP-MRE11 的 U2OS 细胞进行激光诱导 DNA 损伤，然后用 488 nm 激光对 GFP 进行时间序列成像。以 20 秒间隔对细胞成像 6 分钟，以便对 DNA 损伤前后 MRE11 的定位进行可视化和量化。   超级色差校正物镜实现精确的共定位分析 除了研究 MRE11 募集至链断裂位点的动力学过程之外，我们还检查了γH2AX（γH2AX 是一种在 DNA 双链断裂处被磷酸化并激活 DDR 通路的组蛋白）和 CHD4（一种在表观遗传转录

Cell Synchronization by Inhibitors of DNA Replication Induces Replication Stress and DNA Damage Response: Analysis by Flow Cytometry

from the block they synchronously progress through S, G2 , and M. We recently reported that exposure of cells to these inhibitors at concentrations commonly used to synchronize cell populations led to phosphorylation of histone H2AX on Ser 139 (induction of γH2AX

-H2AX Detection in Peripheral Blood Lymphocytes, Splenocytes, Bone Marrow, Xenografts, and Skin

, environmental effects, and aging, as well as in the clinic. DSBs can be detected in the nuclei of cultured cells and tissues with an antibody to H2AX phosphorylated on serine residue 139 (γ-H2AX). DSB levels can be obtained