EZElisa™ DNA定向γ 1（POLg1）试剂盒-EZ

Two-Stage Polymerase Chain Reaction Protocol Allowing Introduction of Multiple Mutations, Deletions, and Insertions, Using QuikChangeTM Site-Directed

(3 ), “megaprimer PCR”(4 –7 ), marker-coupled PCR (8 ), and so on, introducing changes to DNA sequences has become less tedious and more efficient. Recently, marketed site-directed mutagenesis (SDM) kits, such as Transformer™ Site-Directed Mutagenesis

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™

盘点 PCR 的七大应用领域

(2008) An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol.BMC Biotechnol8:91. 6.Xia Y, Chu W, Qi Q et al.(2015) New insights into the QuikChange™ process guide the use of Phusion DNA polymerase