- $280
- LSM BIO
- 进口
- PVT50021
- 2025年07月10日
8 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20℃
- 保质期：
3个月
- 英文名：
CaV2.1(pcDNA6) Plasmid
- 库存：
50
- 供应商：
LSM BIO
- 规格：
2ug
CaV2.1(pcDNA6) Plasmid
Catalog No.: PVT50021
CaV2.1(pcDNA6) PlasmidAlias:Plasmid#26578锛汣acna1a
Gene length:7100bp
Host: mammalian cells
Use(s): Protein expressionFragment Type: ORF
Fragmented species: Mouse
Prokaryotic resistance: Amp
Screening Markers: Blast
Promoter: CMV
replicon: pUC
Copy Number:
Competent cells: Stbl3
Temperature: 37Degrees
Back Bones:
Forward primer:CMV-F
Reverse primer:
Induction method:
Sequence:
gacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaacccactgcttactggcttatcgaaattaatacgactcactatagggagacccaagctggctagcgtttaaacttaagcttggtaccgagctcggatcgatccactagtccagtgtggtggaattcggcttgaggccctttgctctttgcagaatggcccgctttggagacgagatgccgggccgctacggcgcaggcggaggaggctcagggccggccgccggggtggtcgtgggcgccgcgggcggccgaggagccgggggcagccggcagggcgggcagcccggagcgcagaggatgtacaagcagtcgatggcgcagagagcgcggaccatggccctctacaaccccatccctgtccgccagaactgcctcacggtcaaccgctccctgttcctcttcagtgaagacaacgtggtgagaaaatacgccaaaaagatcacggaatggcctcccttcgagtacatgatcctggccaccatcattgctaactgcatcgtcctggccctggagcagcacctccctgatgatgacaagacacccatgtctgagcggctggatgacacggaaccatacttcattggaatcttctgttttgaggctggaattaagatcgtggctcttggctttgccttccacaaaggctcctacctgaggaatggctggaacgtcatggactttgtcgtggtgctaacaggcatcttggccactgtcgggacggagtttgacctacggacactgagggcggttcgtgtgctgcggccactcaagctggtgtctggaatcccaagtttacaagtcgtcctgaagtcaatcatgaaggcgatgatccctctgctgcagatcggcctcctcctgttttttgcaatccttatttttgcaatcatagggttagaattttatatggggaaatttcataccacctgctttgaagaggggacagacgacatccagggtgagtcgccagctccgtgtgggacagaggagcctgcccgcacctgccccaacgggaccaaatgtcagccgtactgggaagggcccaacaacggcatcactcagttcgacaacatcctgtttgctgtgctcactgttttccagtgcatcaccatggaaggctggactgatctcctctacaatagcaacgatgcctcagggaacacttggaactggttgtacttcatccccctcatcatcatcggctccttttttatgctgaaccttgttctgggtgtgctgtctggggagtttgccaaagaaagggaacgtgtggagaaccggagagcttttctgaagctcagaagacaacagcagattgaacgtgagctcaatggatacatggagtggatctcaaaagcagaagaggtgattcttgcggaggacgagacagacgtggagcagaggcacccttttgatggagctcttcggagagctactctgaagaaaagcaagacagacctgctcaaccctgaggaggccgaggaccagctggctgacatcgcgtctgtggggtctcccttcgccagagccagcatcaaaagtgccaagctggagaactcgactttttttcacaagaaggagagaagaatgcgtttctacatccgccgcatggtcaaaactcaggccttctactggaccgtgctcagtctggtagccctcaacacgctgtgcgtcgccattgtccactacaaccagcccgagtggctctccgacttcctctactatgcagaattcattttcttaggactctttatgtccgaaatgtttataaaaatgtacgggctcgggacgcggccttacttccactcttccttcaactgctttgactgtggggtcatcattgggagcatctttgaagtcatctgggctgtcatcaaacccggtacatcctttggaatcagtgtgttacgagctctcaggttattgcgaattttcaaagtcacaaagtactgggcatctctcagaaacctggttgtctccctcctcaactccatgaagtccatcataagtctgttgttcctccttttcctgttcatcgttgtctttgccctcctggggatgcagctgttcggtggccaatttaattttgatgaggggactccgcccaccaacttcgacacttttccagcagcaataatgactgtgtttcagatcctgactggcgaggattggaatgaggtcatgtatgatgggatcaagtctcaggggggcgtgcagggcggcatggtgttctccatctacttcatcgtcctcaccctcttcgggaactacaccctgctgaacgtgttcttggccatcgcagtggacaacctggccaatgcccaggagctcaccaaggatgagcaagaagaggaagaggcagccaatcagaagctggctctacagaaagccaaggaggtagcagaagtgagtcccctgtctgcagccaacatgtccatagccgtgaaggaacagcagaagaaccagaagcctaccaagtcggtgtgggagcagcgcaccagcgagatgcgcaagcagaatctgctggccagccgcgaggcgctgtacggggacgcggctgagcgctggcccaccccctacgcgcgcccgctgcggcccgacgtgaagactcacttggaccggccgcttgtggtggacccgcaggagaaccgtaacaacaacaccaacaagagccgtgcgccagaagcgctgcgcccgaccgcgcggccccgcgagagcgcgcgcgaccccgacgcgcggcgcgcctggcccggcagccctgagcgcgcccctggacgagagggcccgtatgggcgcgagagcgagcctcagcagcgcgatcacgcgccgccccgcgagcacgcaccttgggacgcggacacggagcgcgccaaagccggggacgcgccccgccgccacacgcaccgacctgtggcggagggcgagcctcgccgccaccgcgcccgccgccggcccggggacgaacccgacgacaggccagagcgcaggccgcgtccccgcgacgccactaggccggcccgcgctgcagacggcgaaggcgacgatggggagcgcaagcggcgacaccgacacgggccgcccgcccacgacgacagggagcgcagacaccgacgcagaaaagagaaccagggctctggggtccccgtgtctggtcccaacttgtccaccaccaggccaatccagcaagacctgggccgccaggacctgccgctggctgaggatcttgacaacatgaagaataacaagctggccaccggggaacctgccagtccccacgacagcctgggccacagcggccttccccccagccccgccaaaatcgggaacagcaccaaccctggtcccgccttggccaccaatccccagaacgctgccagccgcaggacgcccaacaatccgggcaacccgtccaaccccggcccccccaagactcccgagaacagccttatcgtcaccaaccccagcagcacccagcccaactcagcgaagactgccaggaaacccgagcacatggcggtggagatccccccagcctgcccgcccctcaaccacaccgtggtccaagtaaacaaaaacgccaacccagacccactgccaaagaaagaggaagagaagaaagaggaagaggaggcagaccccggggaggacggcccaaagcccatgccgccctacagctccatgttcatcctctccaccaccaacccccttcgccggctgtgccattacatcctgaacctgcgctacttcgagatgtgcatcctcatggtcattgccatgagcagcatcgctctggccgctgaggacccggtgcagcccaacgcaccccgcaacaatgtgctgcgatattttgactatgtttttacaggcgtgtttacctttgagatggtgatcaagatgatcgacctgggcctcgtcttgcatcagggggcctatttccgtgacctatggaacattctggacttcatagtggtcagtggggccctggtggcctttgccttcactggcaatagcaaaggaaaggacattaacaccattaagtctctccgagtcctccgggtgctacgacctctaaagaccatcaagcggctgccaaagctgaaggccgtgtttgactgcgtggtgaactcgctcaagaacgtcttcaacatcctcattgtgtacatgctgttcatgttcatcttcgctgtggtagccgtgcagctcttcaagggcaagttcttccactgcacggacgagtccaaggagttcgagagagactgtcgaggcaagtacctcctgtacgagaagaacgaagtgaaggcccgggaccgcgagtggaagaagtacgagttccactacgacaacgtgctctgggctctgctcacacttttcacagtgtccacaggagagggctggccacaggtcctcaagcactcagtggatgctacttttgagaaccagggccccagccccgggtaccgcatggagatgtccatcttctacgtggtgtactttgtagtgtttcccttcttctttgtcaatatcttcgtggccttgatcatcatcacctttcaggagcagggagacaagatgatggaagagtacagcctggagaaaaatgagagggcctgcatcgactttgccatcagtgccaagccgctgaccaggcacatgccccagaacaagcagagcttccagtatcgaatgtggcagttcgtcgtgtccccaccctttgagtacaccatcatggccatgatcgctctcaacaccatcgtgctaatgatgaagttctacggagcctcggtggcctatgaaaacgcccttcgcgtgttcaacattgtcttcacctccctcttctctctcgaatgtgtgcttaaagtcatggcttttgggattctgaattatttccgcgatgcctggaacatcttcgactttgtgactgttctgggcagcatcacagacatcctcgtcaccgagtttgggaataacttcatcaacctgagctttctccgcctcttccgtgctgccagactcatcaaactcctccgccagggttacaccatccgcattctcctctggactttcgtgcagtccttcaaggccctaccttatgtctgtctgctgatcgccatgctcttcttcatctacgccatcatcgggatgcaggtgtttggcaacatcggcattgacggggaagacgaggacagcgacgaggatgaattccaaatcacggagcacaataacttccggaccttcttccaagcgctcatgcttctcttccggagcgccacaggagaagcgtggcacaacatcatgctctcctgcctcagcgggaagccgtgcgataagaactctgggatcctcacggcagactgcggcaacgagttcgcgtacttctactttgtctcgttcatcttcctgtgctcgtttctgatgctgaatctctttgtcgctgtcatcatggacaacttcgagtacctcacccgagattcttccatcctgggcccccatcacttggatgaatacgtgcgtgtctgggcagagtatgaccctgctgcctgcggccgcattcactataaggacatgtacagtttattgcgagtaatatcgccccctctcggcttaggcaagaaatgtcctcatagggttgcttgcaagagactcctgcggatggacctgccggtagcagatgacaacacagttcacttcaactccaccctgatggctctgatccgaaccgcccttgatatcaaaattgccaagggtggagctgacaagcagcagatggatgcagagcttcgcaaggagatgatggccatatggcccaacctgtctcagaagaccctggatctgctggtcacacctcacaagtccacggacctgacagtgggtaagatctacgcagccatgatgatcatggagtattaccggcagagcaaggccaagaaactgcaggccatgcgagaggagcagaaccggacaccactcatgttccagcgcatggagcctccatcaccaacacaggagggaggacccagccagaacgcccttccctccactcagctggacccaggaggaggcctgatggctcacgaaggcggcatgaaggagagcccgtcctgggtgacccagcgggcccaagagatgttccagaagactggcacctggagcccagagcgagggccacccatcgacatgcctaacagccagcccaactcccagtctgtggagatgcgagaaatgggtactgatggctactcagacagcgaacactacctccccatggaaggccagaccagggccgcctccatgccccgcctcccagcagagaaccagaggagaaggggccggccacgtggaaatgacctcagtaccatctctgataccagccccatgaagcgctcagcctctgtgctgggacccaaagcccggcgactggatgactactcactagagcgggtaccgcctgaggagaaccaacggtaccaccaacgtcgccgggaccgtggccaccgcacctctgagcgctctctgggccgatacactgatgtggacacaggtttggggacagatctgagcatgaccacccaatcgggtgacctgccctccaaagatcgggaccaggaccggggccgacccaaggaccggaagcatcggccacaccaccaccaccaccaccaccatcatcatcatcccccggcccccgaccgggatcgctacgcacaggagcggccggacaccggccgggcgcgggcccgggagcagcgctggtcccgctcgcccagcgaaggtcgggagcacacgacacacagacagggcagtagttccgtcagtggaagcccagccccctctacgtccggcaccagcacgccgcggcgcggccgccgccagctcccccagaccccctgcaccccgcggccgcttgtgtcctactcgcccgctccgcgcaggcctgcggcgcgcaggatggcgggtcccgcagcgccccctggcggctcgccgcggggttgccgccgtgcgccgcgctggcctgcgcatgcgcctgagggcccgcggccgcgcggtgccgactacaccgaacccgacagcccgcgcgagccgccagggggcgcgcacgatcccgcgccgcggtcgcccaggactccgcgcgccgcgggctgcgcgtcaccgcgccacggccgcaggctgcccaatggttactacgcggggcacggcgcgccgcggccgcgcacagcccgcaggggcgcgcacgacgcgtacagcgagagcgaggacgactggtgctaagccgccccgccccccgcagcgcccgtaaagtgcgggcgcactgcaaatgataaacaataacgtttttaaaaaagaaaacccgggagaaaaacaaaaatagcttctattgatgagttttatcatctcgattgaatctttcgttttcccggtgaatgaaaccgaggtggctgcaagccggaaggacggctgcacgccccacacacttctaagccgaattatccagcacagtggcggccgctcgagtctagagggcccttcgaaggtaagcctatccctaaccctctcctcggtctcgattctacgcgtaccggtcatcatcaccatcaccattgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattaattctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctctgcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttcggatctgatcagcacgtgttgacaattaatcatcggcatagtatatcggcatagtataatacgacaaggtgaggaactaaaccatggccaagcctttgtctcaagaagaatccaccctcattgaaagagcaacggctacaatcaacagcatccccatctctgaagactacagcgtcgccagcgcagctctctctagcgacggccgcatcttcactggtgtcaatgtatatcattttactgggggaccttgtgcagaactcgtggtgctgggcactgctgctgctgcggcagctggcaacctgacttgtatcgtcgcgatcggaaatgagaacaggggcatcttgagcccctgcggacggtgccgacaggtgcttctcgatctgcatcctgggatcaaagccatagtgaaggacagtgatggacagccgacggcagttgggattcgtgaattgctgccctctggttatgtgtgggagggctaagcacttcgtggccgaggagcaggactgacacgtgctacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgcgatgtacgggccagatatacgcgtt
//
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20渭l ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 渭 L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
Genetic Manipulation of Corynebacterium diphtheriae and Other Corynebacterium Species Author: Chungyu Chang Minh Tan Nguyen Hung Ton‐That First published: 31 August 2020 https://doi.org/10.1002/cpmc.111
CURRENT PROTOCOLS IN MICROBIOLOGY
Volume58, Issue1
September 2020 e111
相关实验
1、质粒的概念 质粒（Plasmid）是一种存在于细菌、酵母等微生物细胞中的小型环状双链 DNA 分子，能够独立于宿主染色体进行自我复制，并通常携带对宿主生存非必需但具有特定功能的基因（如抗生素抗性基因）。质粒是分子生物学和基因工程中最重要的工具之一。 质粒根据其用途可以分为多种不同的类型，如用于保存序列信息的克隆质粒（如：pUC 系列质粒），用于表达蛋白的质粒（如哺乳动物细胞表达所用的 pcDNA3.1 系列质粒），用于基因编辑的质粒（如哺乳动物细胞基因编辑所用的 espCas9-2A
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
the plasmid DNA with very high fidelity. Plasmid DNA is physically independent of genomic DNA and this allows for their easy isolation from bacterial cultures. the pcDNA3.1+ plasmidVector plasmids used for cloning are short, circular, double-stranded DNA
--------------------------------------------- 在啮齿类动物建立尾静脉大容量快速注射方法(也有译为“尾静脉液压注射法”)： 1. Liu F, Song Y, Liu D. 1999. Hydrodynamics-based Transfection in Animals by Systemic Administration of Plasmid DNA. Gene Therapy 6:1258-1266. 2. Zhang G, Budker V and Wolff JA. 1999. High Levels of Foreign
技术资料暂无技术资料 索取技术资料
文献支持
CaV2.1(pcDNA6) Plasmid
$280
