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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20℃
- 保质期：
3个月
- 英文名：
p11L2w Plasmid
- 库存：
50
- 供应商：
LSM BIO
- 规格：
2ug
p11L2w Plasmid
Catalog No.: PVT48915
p11L2w PlasmidAlias:Plasmid #37331
Gene length:
Host: mammalian cells
Use(s): Protein expressionFragment Type: ORF
Fragmented species: Human
Prokaryotic resistance: Kan
Screening Markers: Neo/G418
Promoter: EF1a
replicon: pUC
Copy Number:
Competent cells: DH5a
Temperature: 37Degrees
Back Bones:
Forward primer:
Reverse primer:
Induction method:
Sequence:
ccgctaccggactcagatctcgagctcaagcttgcaaagatggataaagttttaaacagagaggaatctttgcagctaatggaccttgtaggtcttgaaaggagtgggaattggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggtgtcgtgaggaattctctagatgaagcccagagcccggcggagaaagcgggccagcgccacccagctgtaccagacctgcaaggccaccggcacctgcccccctgacgtgatccccaaggtggagcacaccaccatcgccgaccagatcctgaagtggggcagcctgggcgtgttcttcggcggcctgggcatcggcaccggcgccggcagcggcggccgggccggctacatccccctgggcagcagccctaagcccgccatcaccgggggccctgccgccagaccccccgtgctggtggagcctgtggcccccagcgaccccagcatcgtgagtctgatcgaggagagcgccatcatcaacgccggcgcccctgaggtggtgcctcctacccagggcggcttcaccatcaccagcagcgagagcaccacccctgccatcctggacgtgagcgtgaccaaccacaccaccaccagcgtgttccagaaccccctgttcaccgagcctagcgtgatccagccccagcctcccgtggaggccagcggccacatcctgatcagcgcccctaccatcaccagccagcacgtggaggacatccccctggacaccttcgtggtgagcagcagcgacagcggccccaccagcagcacccctctgccccgggccttccccagacctcgggtgggcctgtacagcagagccctgcagcaggtgcaggtgaccgaccccgccttcctgagcaccccccagcggctggtgacctacgacaaccctgtgtacgagggcgaggacgtgagcctgcagttcacccacgagagcatccacaacgcccccgacgaggccttcatggatatcatcagactgcaccggcccgccatcaccagcagacggggcctggtgcgcttcagcagaatcggccagcggggcagcatgtacacccggagcggccagcacatcggcgccagaattcactacttccaggacatcagccctgtgacccaggccgccgaggagatcgagctgcaccccctggtggccgccgagaacgacaccttcgacatctacgccgagcccttcgaccctatccccgaccccgtgcagcacagcgtgacccagagctacctgaccagcacccctaacaccctgagccagagctggggcaacaccaccgtgcccctgagcatccccagcgactggttcgtgcagagcggccccgacatcaccttccctaccgctagcatgggcacccccttcagccccgtgacccctgccctgcccaccggccccgtgttcatcaccggcagcgacttctacctgcaccctacctggtacttcgcccggcggagacggaagcggatccccctgttcttcaccgacgtggccgcctaagcttatcgataccgtcatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccgtcggccgcttccctttagtgagggttaatgcttcgagcagacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtatggaattcttaattaactagccatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttcttaattaagctgtacactgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatgactgactaattttttttatttatgcagaggccgaggccgcctctgcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttcggatctgatcagcacgtgttgacaattaatcatcggcatagtatatcggcatagtataatacgactcactataggagggccaccatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacacatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgaattcgctagagggaggctaactgaaacacggaaggagacaataccggaaggaacccgcgctatgacggcaataaaaagacagaataaaacgcacggtgttgggtcgtttgttcataaacgcggggttcggtcccagggctggcactctgtcgataccccaccgagaccccattggggccaatacgcccgcgtttcttccttttccccaccccaccccccaagttcgggtgaaggcccagggctcgcagccaacgtcggggcggcaggccctgccatag
//
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20渭l ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 渭 L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
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文献和实验该产品被引用文献
Genetic Manipulation of Corynebacterium diphtheriae and Other Corynebacterium Species Author: Chungyu Chang Minh Tan Nguyen Hung Ton‐That First published: 31 August 2020 https://doi.org/10.1002/cpmc.111
CURRENT PROTOCOLS IN MICROBIOLOGY
Volume58, Issue1
September 2020 e111
相关实验
Large-Scale Isolation of Plasmid DNA
实验步骤 1. Inoculate 200 ml of L2 containing appropriate selective agent with a single colony or culture containing the desired plasmid. Grow at 37 degrees overnight. 2. Spin down cells at 5000 rpm for 15 minutes
This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
Plasmid Profile Typing and Plasmid Fingerprinting
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids
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p11L2w Plasmid
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