- ¥2935.90
- 阿拉丁
- 上海
- D128544
- 2026年05月08日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
避光,-20°C储存；超低温运输
- 英文名：
Diaphorase from Clostridium kluyveri
- 库存：
现货
- 供应商：
上海阿拉丁生化科技股份有限公司
- 规格：
D128544-2KU
基本描述
产品名称：心肌黄酶 来源于梭状芽孢杆菌
规格或纯度：EnzymoPure™, ≥25 units/mg dry weight
产品介绍：
Diaphorase catalyzes the reaction of a reduced di- or tri-phosphopyridine nucleotide hydrogen donor with a hydrogen acceptor, usually a dye in the leucoform.Diaphorase from Clostridium kluyveri, or Lipoyl dehydrogenase, has been used in a study to assess the protein-protein interactions in assembly of lipoic acid on the 2-oxoacid dehydrogenases of aerobic metabolism. Lipoyl dehydrogenase has also been used in a study to investigate the redox regulation of tyrosine nitration and 3-nitrotyrosine reduction by antioxidants.
级别：EnzymoPure™
产品规格参数
浓度：≥25 units/mg dry weight
储存条件：避光,-20°C储存
运输条件：超低温运输
CAS编号和信息：9001-18-7
酶学委员会编号：1.6.99.1
单位定义：1 Unit reduces 1µmole of Dichlorophenolindophenol (DCPIP) per minute at 25°C, pH 8.5.
功能和特点
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文献和实验SEMI-DRY ELECTROPHORETlC TRANSFER (WESTERN BLOTS)
. Carefully disassemble the stack, placing the membrane on dry Kimwipes and store at room temperature*. The polyacrylamide gel can now be stained to verify transfer. Pre-stained molecular weight standards run on the gels should transfer to the nitrocellulose
A protocol for cleaning and reusing the large 25 x 25 cm plates
We regularly reuse our large 25x25 cm plating trays; initially, however, we were plagued by gross microbiological contamination when reusing the trays. Various combinations of cleaning with a laboratory dishwasher, ethyl alcohol, and UV
Dry Transfer Trans-Bot SD Assembly 1. Prepare the transfer buffer. 2. Following electrophoresis, equilibrate the gels in transfer buffer. Equilibration facilitates the removal of electrophoresis buffer salts and detergents.
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