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- 文献和实验
- 技术资料
- 保存条件:
2-8°C储存;低温运输
- 英文名:
Trypsin from bovine pancreas
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
T128774-100mg/T128774-1g/T128774-5g
| 规格: | T128774-100mg | 产品价格: | ¥410.90 |
|---|---|---|---|
| 规格: | T128774-1g | 产品价格: | ¥2199.90 |
| 规格: | T128774-5g | 产品价格: | ¥9769.90 |
基本描述
产品名称:胰蛋白酶 来源于牛胰腺
规格或纯度:EnzymoPure™, ≥180 units/mg protein
产品介绍:
Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. It is derived from a 34 kDa inactive precursor zymogen, trypsinogen, after enzymatic removal of an N-terminal 6-amino acid leader sequence resulting in the 23.8 kDa trypsin molecule. The optimum pH is 8.0. Trypsin is inhibited by organophosphorus compounds such as diisopropylfluorophosphate and natural inhibitors from pancreas. Soybean, lima bean, and egg white are also sources of natural inhibitors. Trypsin cleaves amide and ester bonds of Arg and Lys. The Aladdin Sequencing Grade Trypsin has been further purified to remove trace contaminating proteases and autolysis products which could interfere in trypsin digestion experiments, and exhibits a single band on PAGE.Trypsin is a serine protease used to hydrolyze proteins. Trypsin from bovine pancreas has a molecular weight of 23.8 kDa. Trypsins are used for the re-suspension of cells during cell culture and in proteomics research for the digestion of various proteins
Accession #:
级别:EnzymoPure™
产品规格参数
浓度:≥180 units/mg protein
储存条件:2-8°C储存
运输条件:低温运输
CAS编号和信息:9002-07-7
酶学委员会编号:3.4.21.4
单位定义:在25℃,ph 8.2,10mm 钙存在下,每分钟水解1μmole 对甲苯磺酰 l 精氨酸甲酯
功能和特点
Accession #:
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文献和实验(1 mg will inhibit 1 mg of trypsin with an activity of 10,000 BAEE units/mg protein). T6552 is of higher purity (1 mg will inhibit 1.0-3.0 mg ot trypsin with an activity of 10,000 BAEE units/mg protein), but more importantly, it has been suitability
Purification of Antiserum or Ascites by Protein A/G Chromatography
A and protein G bind about 20 mg of IgG per ml of conjugated agarose. Therefore, the binding capacity of a 10 ml (5 ml) column is 200 mg (100 mg) of IgG. A high-titer rabbit antiserum has roughly 5 mg/ml of IgG, mouse ascites has roughly 10 mg/ml of IgG
Application of Amide Proton Exchange Mass Spectrometry for the Study of Protein‐Protein Interactions
one 13 C atom appears one mass unit to the right of the monoisotopic peak, the peak representing the fraction containing two 13 C atoms appears two mass units to the right of the monoisotopic peak, and so on. (B ) Sequence coverage map for the protein
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