胰蛋白酶 来源于牛胰脏，9002-07-7，EnzymoPu

产品名称：胰蛋白酶 来源于牛胰脏

规格或纯度：EnzymoPure™, potency ≥3000 units/mg

产品介绍：

胰蛋白酶是一种丝氨酸蛋白水解酶,属白色或微黄色冻干粉制品。它能特异性地水解碱性氨基酸精氨酸及赖氨酸羧基所组成的肽键。酶本身很容易自溶，其作用最佳的PH值为8.0～9.0。胰蛋白酶活性能被胰腺中的天然抑制剂所抑制。钙离子能延迟胰蛋白酶的自溶，并能促进胰蛋白酶原的活性。 or trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestionsns?. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.

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级别：EnzymoPure™

浓度：potency ≥3000 units/mg

储存条件：-20°C储存

运输条件：超低温运输

CAS编号和信息：9002-07-7

酶学委员会编号：3.4.21.4

单位定义：一个BAEE单位以BAEE作为底物，在25°C、pH 7.6条件下每分钟每毫升产生0.001的ΔA253 。一个BTEE单位=320个ATEE单位。反应体积=3.2mL（1cm的光径）。

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