- ¥231.90 - 10668.90
- 阿拉丁
- 上海
- C128708
- 2026年10月16日
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- 文献和实验
- 技术资料
- 保存条件：
2-8°C储存；低温运输
- 英文名：
Collagenase from Clostridium histolyticum(Type 3)
- 库存：
现货
- 供应商：
上海阿拉丁生化科技股份有限公司
- 规格：
C128708-1g/C128708-250mg/C128708-5g/C128708-50mg
| 规格： | C128708-1g | 产品价格： | ¥2667.90 |
|---|---|---|---|
| 规格： | C128708-250mg | 产品价格： | ¥925.90 |
| 规格： | C128708-5g | 产品价格： | ¥10668.90 |
| 规格： | C128708-50mg | 产品价格： | ¥231.90 |
基本描述
产品名称：胶原蛋白酶 来源于溶组织梭菌(类型3)
规格或纯度：EnzymoPure™, ≥100 units/mg dry weight
产品介绍：
Crude collagenase preparations contain several isoforms of two different collagenases, a sulfhydryl protease, clostripain, a trypsin-like enzyme, and an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential part of tissue dissociation. One component of the complex is a hydrolytic enzyme which degrades the helical regions in native collagen preferentially at the Y-Gly bond in the sequence Pro-Y-Gly-Pro, where Y is most frequently a neutral amino acid. This cleavage yields products susceptible to further peptidase digestion. Crude collagenase is inhibited by metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by α2-macroglobulin, a large plasma glycoprotein. Ca2+ is required for enzyme activity. Particular enzymatic profiles of each collagenase have been correlated with the tissues from which the cells for study were obtained (or with the uses to which the cells are put) and as a result of the correlations several types of crude collagenases have been established by Aladdin: Types 1, 2, 3, and 4.This collagenase has been tested with cell lines to verify the product is not cytotoxic. Collagenase is typically used to digest the connective components in tissue samples to liberate individual cells. The concentration for cartilage dispersal is 1-2 mg/ml, but literature searches should be performed for species specific and/or tissue specific concentrations.
Accession #：
级别：EnzymoPure™
产品规格参数
浓度：≥100 units/mg dry weight
储存条件：2-8°C储存
运输条件：低温运输
CAS编号和信息：9001-12-1
功能和特点
Accession #：
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文献和实验High Molecular Weight Yeast Liquid DNA Preparation
Purpose: To isolate intact, high molecular weight DNA from yeast cells for subcloning and rare cutting restriction enzyme analysis. One can expect a yield of 100-200 � of DNA per prep. Time required: Day
How to Make Simple Solutions and Dilutions
= 200 ul = 0.2 ml C2 = the new concentration = 25 mg/ ml By algebraic rearrangement: V1 = (V2 x C2) / C1 V1 = (0.2 ml x 25 mg/ml) / 100 mg/ml and after cancelling the units, V1 = 0.05 ml, or 50 ul
How to Make Simple Solutions and Dilutions
concentration = 200 ul = 0.2 ml C2 = the new concentration = 25 mg/ ml By algebraic rearrangement: V 1 = (V 2 x C 2 ) / C 1 V 1 = (0.2 ml x 25 mg/ml) / 100 mg/ml and cancel the units
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