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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20°C储存
- 英文名:
Proteinase K
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
P109033-5g/P109033-500mg/P109033-25mg/P109033-25g/P109033-1g/P109033-100mg/P109033-100g
| 规格: | P109033-5g | 产品价格: | ¥2390.9 |
|---|---|---|---|
| 规格: | P109033-500mg | 产品价格: | ¥330.9 |
| 规格: | P109033-25mg | 产品价格: | ¥51.9 |
| 规格: | P109033-25g | 产品价格: | ¥9785.9 |
| 规格: | P109033-1g | 产品价格: | ¥598.9 |
| 规格: | P109033-100mg | 产品价格: | ¥102.9 |
| 规格: | P109033-100g | 产品价格: | ¥30797.9 |
英文名:Proteinase K
纯度或规格:EnzymoPure™, 冻干粉,≥30 units/mg protein
SPU:P109033
CAS:39450-01-6
存储温度:-20°C储存
运输条件:超低温运输
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
INTRODUCTION The formidable size and structure of polytene chromosomes allow mapping of chromosomal protein distributions at very high resolution. This protocol describes the preparation of polytene chromosomes from Drosophila larvae
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
-galactopyranoside (X-gal; 25 mg/mL in DMF [dimethylformamide]) Agarose gel (1%, UltraPure; Invitrogen) Antibody, specific for the protein of interest Bead eluting buffer Bovine serum albumin (BSA; 0.5% in PBS; New England Biolabs) Cell
Thrombin Cleavage of GST-Fusion protein
to cleave 1 mg of fusion protein at room temperature overnight. 1 X PBS buffer: 140mM NaCl; 2.7mM KCL; 10mM Na2HPO4; 1.8mM KH2PO4; pH 7.3 Thrombin Cleavage of Free Eluted Fusion protein First you have to calibrate amount of thrombin, temperature
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