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- 文献和实验
- 技术资料
- 保存条件:
-20°C储存,避免反复冻融;超低温运输
- 英文名:
Rat Serum
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
R501133-100ml/R501133-10ml
| 规格: | R501133-100ml | 产品价格: | ¥2399.90 |
|---|---|---|---|
| 规格: | R501133-10ml | 产品价格: | ¥399.90 |
中文名:大鼠血清
英文名:Rat Serum
纯度:BioReagent,无菌,>55 mg protein/mL,0.22 µm filtered
货号:R501133
存储温度:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性存储:Store at -20℃ for 5 years. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
Native Chromatin Preparation and Illumina/Solexa Library Construction
. Extract each tube once with 200 µL of phenol:chloroform. iv. Add 2 µL of 20 mg/mL glycogen, 20 µL of 3 M NaOAc (pH 5.3), and 500 µL of ethanol to each tube to precipitate the DNA. 36. Wash each pellet
at 4°CEthidium bromide solution (20 mg/ml in H2O) (for duplex PCR only; see Steps 40-53)Forward and reverse primers (100 µM stock solutions in H2O)For duplex PCR (Steps 40-53), primers should be designed in order to obtain comparable amplifications
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