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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
R3/I5
- 库存:
期货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
rp174869-500μg/rp174869-1mg
| 规格: | rp174869-500μg | 产品价格: | ¥11999.9 |
|---|---|---|---|
| 规格: | rp174869-1mg | 产品价格: | ¥19999.9 |
设定黄金标准,在阿拉丁,我们不仅相信保持质量,而且相信质量。 我们的目标是定义它。 我们的质量管理理念为业界研究试剂建立了高质量的标准。 当您听到阿拉丁这个名字时,就会知道它是各个方面坚定不移品质的灯塔。
中文名:R3/I5
英文名:R3/I5
纯度:Moligand™
cas号:-
存储温度:-
运输条件:-
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文献和实验Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
(most critical timed step). 5. Add 300 ul of cold Qiagen buffer R3 (neutralization buffer) per well. Seal wells with plastic sealing tape and mix by gentle inversion 5 times. Incubate on ice for 5 minutes. 6. Add 50 ul of ProCipitate™ (LigoChem
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
system such as the Qiagen BioRobot 9600. When doing so, R1, R3, and isopropanol are at ambient temperature and the ProCipitate™ step is excluded. 【2】G-force is limited by the centrifuge available. Centrifuges which allow higher g-force can be used to reduce centrifugation
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmids*
. Alternatively, 24 deep-well or 48 deep-well blocks may be used with up to 4.8 or 2.4ml of media respectively. Cover each block with an AirPore™ (Qiagen Inc.) sheet and incubate at 37�C with shaking at 325 RPM for approximately 20 hours. 2. Pellet cells
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