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- 文献和实验
- 技术资料
- 保存条件:
室温
- 英文名:
R3/I5
- 库存:
期货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
rp174869-1mg/rp174869-500μg
| 规格: | rp174869-1mg | 产品价格: | ¥19999.90 |
|---|---|---|---|
| 规格: | rp174869-500μg | 产品价格: | ¥11999.90 |
基本描述
产品名称:R3/I5
规格或纯度:Moligand™
级别:Moligand™
作用类型:激动剂
作用机制:RXFP3 的激动剂;RXFP4 的激动剂
产品规格参数
储存条件:室温
CAS编号和信息:rp174869
功能和特点
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文献和实验Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
(most critical timed step). 5. Add 300 ul of cold Qiagen buffer R3 (neutralization buffer) per well. Seal wells with plastic sealing tape and mix by gentle inversion 5 times. Incubate on ice for 5 minutes. 6. Add 50 ul of ProCipitate™ (LigoChem
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
system such as the Qiagen BioRobot 9600. When doing so, R1, R3, and isopropanol are at ambient temperature and the ProCipitate™ step is excluded. 【2】G-force is limited by the centrifuge available. Centrifuges which allow higher g-force can be used to reduce centrifugation
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmids*
. Alternatively, 24 deep-well or 48 deep-well blocks may be used with up to 4.8 or 2.4ml of media respectively. Cover each block with an AirPore™ (Qiagen Inc.) sheet and incubate at 37�C with shaking at 325 RPM for approximately 20 hours. 2. Pellet cells
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