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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
避光;室温
- 英文名:
Indeno(1,2,3-cd)pyrene solution
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
193-39-5
- 规格:
I301711-2ml
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文献和实验1.5M Tris(PH 8.8) 100ml 18.171g (at4°C) 10% SDS 100 ml 10g (at room temperature) 10% ammonium persulfate 1ml 0.1g(replace every week store at at4°C) TEMED 8 room temprature Antibodies Primary antibody: 5A11(anti-mouse IgG) 500x-1000x Secondary
200 ml Methanol600 ml H20-- cut fresh nitrocellμlose (11 X 17 cm)& Whatman soak in TB-- 2 grids outside plate on Yellow Tape side1 grid in middle SMOOTH SIDE TO GEL!Cushionspour in TB until just wetput 1 Whatman over Gel à smooth outplace
RNA in situ protocol for DNA and RNA probes
Other reagents needed: Drosophila saline (0.7% NaCl; 0.03% Triton X-100) 50% bleach heptane methanol absolute ethanol (EtOH) xylene 5 mgs/ml proteinase K PBT + 2 mgs/ml glycine PBT + 50 mM EDTA nitroblue tetrazolium (NBT) (75 mgs/ml in DMF) X
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