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- 文献和实验
- 技术资料
- 保存条件:
室温;常规运输
- 英文名:
Thiocyclam-hydrogen oxalate solution
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
31895-22-4
- 规格:
T114803-1ml
中文名:杀虫环标准溶液
英文名:Thiocyclam-hydrogen oxalate solution
纯度:分析标准品,10ug/ml in methanol
货号:T114803
Cas号:31895-22-4
存储温度:室温
运输条件:常规运输
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文献和实验Yeast IF with Methanol/Acetone Dehydration
containing 1.2M sorbitol. Be very gentle with the cells! Resuspend in 0.5 mls sorbitol buffer. The cells can be stored at this point overnight at 4¡C. Zymolyase treat the cells with 30 ul 10 mg/ml Zymolyase (100T) at 30¡C for anywhere between 10-30 min
Yeast IF with Methanol/Acetone Dehydration
in 0.5 mls sorbitol buffer. The cells can be stored at this point overnight at 4¡C. Zymolyase treat the cells with 30 ul 10 mg/ml Zymolyase (100T) at 30¡C for anywhere between 10-30 min or more. Examine cells on a phase microscope. When cells are dark
1.5M Tris(PH 8.8) 100ml 18.171g (at4°C) 10% SDS 100 ml 10g (at room temperature) 10% ammonium persulfate 1ml 0.1g(replace every week store at at4°C) TEMED 8 room temprature Antibodies Primary antibody: 5A11(anti-mouse IgG) 500x-1000x Secondary
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