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- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant human Lyn protein fragments expressed in Ecoli (AA 8-238)
- 亚型:
Mouse IgG1
- 保存条件:
-20°C储存,避免反复冻融;超低温运输
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 浓度:
1.0 mg/mL
- 规格:
Ab113790-100μl/Ab113790-1ml/Ab113790-50μl/Ab113790-10μl
| 规格: | Ab113790-100μl | 产品价格: | ¥1574.90 |
|---|---|---|---|
| 规格: | Ab113790-1ml | 产品价格: | ¥16999.90 |
| 规格: | Ab113790-50μl | 产品价格: | ¥974.90 |
| 规格: | Ab113790-10μl | 产品价格: | ¥499.90 |
基本信息
产品名称:Lyn Mouse mAb
规格或纯度:ExactAb™, Validated, 1.0 mg/mL
应用:WB
特异性:LYN
种属反应性:人(Human)
免疫原:Purified recombinant human Lyn protein fragments expressed in Ecoli (AA 8-238)
阳性对照:WB: Ramos, Raji, K562, HeLa and A431 cell lysates.
偶联:Unconjugated
产品属性
克隆类型:单克隆抗体
克隆号:1C8-H3-G6
Format:Whole IgG
亚型:Mouse IgG1
SDS-PAGE:150 kDa
纯化方法:Protein G purified
物理外观:Liquid
储存缓冲液:10mM PBS, 50% Glycerol, 0.5% BSA, 0.02% Sodium azide, pH 7.3
防腐剂:0.02% Sodium azide
浓度:1.0 mg/mL
储存条件:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性与储存:4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属:小鼠(Mouse)
种属反应性:人(Human)
克隆号:1C8-H3-G6
偶联:Unconjugated
图片
>
Lyn Mouse mAb (Ab113790) - Western Blot
All lanes: Lyn Mouse mAb (Ab113790) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 59 kDa
Observed band size: 55 kDa
Exposure time: 47.5 s
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文献和实验Characterization and Validation of Cre‐Driver Mouse Lines
animals Ppm1a‐CreERT2 /RXRαΔAF2(LNL) and Vil1‐CreERT2 /RXRαΔAF2(LNL) mice injected with vehicle (top) or with tamoxifen (bottom). For tamoxifen injections, tamoxifen (Sigma, cat. no. T56648) was prepared at 10 mg/ml in sunflower seed oil (Sigma, cat. no. S
) MTT (Sigma, cat. no. M5655‐1G) dissolved in PBS at 5 mg/ml. Should be stored in dark and room lights should be off, while working with MTT. DMSO (Fisher, cat. no. D128
) corresponds to about 50µg/ml of chromatin DNA. So the yield of chromatin (in µg) is given by A260 x dilution factor x volume x 50; Centrifuge sample (800 x g for 10 minutes at 4°C) and resuspend pellet to a chromatin DNA concentration of 0.5mg/ml
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