Recombinant CLIC4 Antibody，重组,

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Dissolve in buffer A the purified antibody carrying an engineered phosphorylation site (e.g., the sequence DDDSDDDD; at 1 mg/ml concentration (alternatively, exchange buffer by dialysis). To 100 µl antibody solution, add 500 units casein

Differentiating Neural Stem Cells into Neurons and Glial Cells

of the secondary antibody host species, 1% BSA, 0.1% Triton®-X in D-PBS with Ca2 and Mg2 ). Note: If you are using a surface antigen such as GalC, omit Triton®-X from the blocking buffer.        b. Remove the blocking buffer and incubate the cells overnight

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minutes at 4°C. Discard the supernatant. 3. Dissolve the pellet in 1 ml of lysate buffer. Bring to 1 mM PMSF by adding 100 mM PMSF stock (17.42 mg/ml in methanol). This solution may be kept for 1 week at -20°C. 4. Add 50 µl of a 10 mg/ml lysozyme