- ¥499.90 - 16999.90
- 阿拉丁
- 上海
- Ab096857
- 2026年05月09日
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide derived from human CLIC4 (AA 3-16)
- 亚型：
Rabbit IgG
- 保存条件：
-20°C储存,避免反复冻融；超低温运输
- 库存：
期货
- 供应商：
上海阿拉丁生化科技股份有限公司
- 浓度：
0.5 mg/mL
- 规格：
Ab096857-100μl/Ab096857-10μl/Ab096857-1ml/Ab096857-50μl
| 规格： | Ab096857-100μl | 产品价格： | ¥2099.90 |
|---|---|---|---|
| 规格： | Ab096857-10μl | 产品价格： | ¥499.90 |
| 规格： | Ab096857-1ml | 产品价格： | ¥16999.90 |
| 规格： | Ab096857-50μl | 产品价格： | ¥1299.90 |
基本信息
产品名称：Recombinant CLIC4 Antibody
规格或纯度：重组, ExactAb™, Validated, KD, 0.5 mg/mL
应用：WB, IF/ICC, IHC
特异性：CLIC4
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat)
免疫原：A synthetic peptide derived from human CLIC4 (AA 3-16)
阳性对照：WB: HeLa, HEK293, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Human Cholangiocarcinoma tissue. ICC/IF: HeLa cells.
偶联：Unconjugated
产品属性
克隆类型：重组抗体
Format：Whole IgG
亚型：Rabbit IgG
SDS-PAGE：150 kDa
纯化方法：Antigen affinity purified
物理外观：Liquid
储存缓冲液：50mM Tris-Glycine, 150mM NaCl, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.4
防腐剂：0.01% Sodium azide
浓度：0.5 mg/mL
储存条件：-20°C储存,避免反复冻融
运输条件：超低温运输
稳定性与储存：4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属：兔(Rabbit)
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat)
偶联：Unconjugated
图片
Recombinant CLIC4 Antibody (Ab096857) - Western Blot
All lanes: Recombinant CLIC4 Antibody (Ab096857) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 28 kDa
Exposure time: 21.9 s
Recombinant CLIC4 Antibody (Ab096857) - Western Blot
All lanes: Recombinant CLIC4 Antibody (Ab096857) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 28 kDa
Exposure time: 35.0 s
Recombinant CLIC4 Antibody (Ab096857) - IF/ICC
Immunocytochemistry analysis of CLIC4 (green) in HeLa using Recombinant CLIC4 Antibody (Ab096857), and cells were counterstained with DAPI (blue).
Recombinant CLIC4 Antibody (Ab096857) - IHC
Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant CLIC4 Antibody (Ab096857). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant CLIC4 Antibody (Ab096857) - Western Blot
All lanes: Recombinant CLIC4 Antibody (Ab096857) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 28 kDa
Exposure time: 0.5 s
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文献和实验Dissolve in buffer A the purified antibody carrying an engineered phosphorylation site (e.g., the sequence DDDSDDDD; at 1 mg/ml concentration (alternatively, exchange buffer by dialysis). To 100 µl antibody solution, add 500 units casein
Differentiating Neural Stem Cells into Neurons and Glial Cells
of the secondary antibody host species, 1% BSA, 0.1% Triton®-X in D-PBS with Ca2 and Mg2 ). Note: If you are using a surface antigen such as GalC, omit Triton®-X from the blocking buffer. b. Remove the blocking buffer and incubate the cells overnight
minutes at 4°C. Discard the supernatant. 3. Dissolve the pellet in 1 ml of lysate buffer. Bring to 1 mM PMSF by adding 100 mM PMSF stock (17.42 mg/ml in methanol). This solution may be kept for 1 week at -20°C. 4. Add 50 µl of a 10 mg/ml lysozyme
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