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- 文献和实验
- 技术资料
- 保存条件:
-20°C储存,避免反复冻融;超低温运输
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 应用范围:
ELISA,CLIA,LFAs,WB and other immunoassay methods
- 浓度:
见COA
- 规格:
Ab170447-10mg/Ab170447-1mg/Ab170447-5mg
| 规格: | Ab170447-10mg | 产品价格: | ¥1199.90 |
|---|---|---|---|
| 规格: | Ab170447-1mg | 产品价格: | ¥229.90 |
| 规格: | Ab170447-5mg | 产品价格: | ¥799.90 |
基本信息
产品名称:Goat Anti-Human IgG Antibody
规格或纯度:已验证,PBS Only,见COA
应用:WB, ELISA, CLIA, LF
应用:ELISA,CLIA,LFAs,WB and other immunoassay methods
种属反应性:人(Human)
偶联:Unconjugated
产品属性
克隆类型:多克隆抗体
Format:Whole IgG
纯化方法:ntigen affinity chromatography
物理外观:liquid
储存缓冲液:10mM phosphate buffered saline (PBS), pH7.4
浓度:见COA
储存条件:-20°C储存,避免反复冻融
运输条件:超低温运输
纯度:≥ 95%
功能和特点
宿主种属:山羊(Goat)
种属反应性:人(Human)
偶联:Unconjugated
图片
Goat Anti-Human IgG Antibody (Ab170447) - ELISA
Goat Anti-Human IgG Antibody (Ab170447) was tested by ELISA. The plate was coated with different amounts of human IgG (Ab170213) (3-fold started from 1000 ng/mL. Goat Anti-Human IgG Antibody (Ab170447) was then added at a series of different dilution ratios. Rabbit-Anti-Goat IgG H&L HRP (Ab141534) was used at 1/10000 dilution as the secondary antibody.
Goat Anti-Human IgG Antibody (Ab170447) - ELISA
Cross-reactivity of Goat Anti-Human IgG Antibody (Ab170447) was tested by ELISA. The wells were coated with the indicated IgG standards at 1 µg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Goat Anti-Human IgG Antibody was then added at different dilution ratio followed by incubation for 1h. Rabbit-Anti-Goat IgG H&L HRP (Ab141534) was used at 1/10000 dilution as the secondary antibody.
For the batch tested, Goat Anti-Human IgG Antibody (Ab170447) showed a cross-reactivity with rabbit IgG when the dilution ratio of Rabbit anti-mouse IgG antibody is less than 1/8000.
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文献和实验-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
Anti-GFP/BrdU In Situ Immunostaining
Materials 24 well dishes Millicell 0.4 µm inserts (Millipore PICM01250) - coated with fibronectin Culture medium PBS Fixative: 4% paraformaldehyde in PBS (pH 7.6) Rabbit anti-GFP (Molecular Probes, A-11122) FITC-goat anti-rabbit
antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000 - 1:20,000 dilution in 4% BSA in TBST. This should be titrated for optimal results. I incubate with primary Ab 3 hours - overnight at room temp with shaking.10. Remove the antibody solution
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