tetralin_urea analogue (7o)，Mo

二苯脲diphenyl urea

1 ， 3 －二苯脲及 N ， N ′－二苯脲。 C 13H 12N 2O ，是从椰子汁中分离测定出的化合物，也有人怀疑它是提取操作中混入的非天然化合物。具有象细胞分裂素那样的生理作用，其活性比普通的细胞分裂素较弱。在合成的衍生物中，也有相当强的活性化合物，例如 N － 3 氯苯 -N ′ - 苯脲， N － 4- 硝基苯 -N ′ - 苯脲等。此外，从人尿中还分离出具有次脲基（ NH － CO － NH ）的嘌呤衍生物 N- （ purine － 6-ylcarbamoyl

Overview of Alternative Oligonucleotide Chemistries for Exon Skipping

by RNA analogues having 2′-O-substitution and a phosphorothioate linkage and charge-neutral backbones such as PNA and PMO. Also discussed are peptide conjugates of PNA and PMO that enhance cellular and in vivo delivery and their potential for drug

Sample preparation (preparative gels)

were shaken overnight at 4o C. After washing with PBS, the cells were incubated with fluorescein-conjugated anticytokeratin antibodies (CAM 5.2) for 45 min and then sorted by FACS. The pellet was mixed with 100 µl per 106 cells of a solution containing urea